Holidic Media for Drosophila
Chemically defined diet for precise nutritional control in feeding experiments. Enables manipulation of individual amino acids, sugars, and other nutrients.
Resources
Experiment Protocols
Standardized protocols for every Easy Behavior system. Follow published methods to ensure reproducible results across experiments and labs.
Equipment Protocols
Standard operating procedures for each Easy Behavior system.
flyPAD Feeding Assay
Standard single-food or two-choice feeding assay for Drosophila melanogaster. Covers fly preparation, food loading, arena setup, data acquisition, and analysis pipeline.
flyPAD Food Intake
Two-Choice Preference Assay
Protocol for measuring food preference between two options. Includes arena preparation with two food wells, preference index calculation, and controls for side bias.
flyPAD Food Preference
optoPAD Closed-Loop Protocol
Setting up behavior-contingent optogenetic stimulation during feeding. Covers LED calibration, Bonsai workflow configuration, trigger thresholds, and Gal4/UAS cross preparation.
optoPAD Optogenetics
flyGym Exercise Protocol
Controlled exercise regime protocol for Drosophila. Covers loading flies, setting exercise intensity and duration, rest intervals, and long-term exercise study design.
flyGym Exercise
Sample Preparation Protocols
Published protocols for fly food and colony maintenance relevant to behavioral assays.
Axenic (Germ-Free) Drosophila
Generating germ-free flies for microbiome studies. Essential for experiments investigating the role of commensal bacteria in feeding behavior and food preference.
Starvation Protocols
Standardized starvation conditions for hunger-motivated feeding assays. Covers timing, water access, and controls for consistent motivational state.
Need a custom protocol?
Contact us to discuss protocol adaptations for your specific experimental needs.